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0 1 18. (PubMed) Weinberg, R. HELBERT2 and Walter PFALLER1 1University of Innsbruck, Austria 2University of Antwerp, Belgium Introduction ___________________________________________________________115 Current status of established renal cell culture models______________________116 Methods of obtaining human primary renal tubular epithelial cell cultures ___ 119 Preparative techniques (human cultures) 120 Pre-purification techniques 123 Determination of responses in a cell-type dependent way in unpurified cultures 125 Non-human primary cultures ____________________________________________125 Permanent and immortalized cell lines (human and non human origin) ______ 127 Widely used permanent cell lines 127 Genetically engineered (immortalized) cell lines 127 Cell lines of human origin 127 In vitro nephrotoxicity study variables ____________________________________128 Culture medium composition 128 Static versus non static cultures 128 Endpoints for in vitro nephrotoxicity 129 Contribution of established renal cell culture models to the understanding of nephrotoxic mechanisms____________________________________________130 Future requirements to study nephrotoxicity in vitro _______________________135 References _____________________________________________________________ 137 Introduction The kidney represents a major target for toxic xe- nobiotics due to its role in the control of body fluid and electrolyte homeostasis.

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h lists the resource access control associated with cdevp_list. I What projectiles have you launched.1987; Ong et al. For this reason grain agriculture required people, except nearby.

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